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The Culling Game arc itself. He says he drew it so messily that he regrets not making it a tournament arc instead and including Gojo's rescue.

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Regarding Megumi's sister. Since he killed her off without any interaction with Itadori, readers didn't really feel sad about her death. He regrets not building a connection with Itadori before killing her off.

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Kirara Hoshi (male)'s technique. He regrets making the ability too complex and wishes he'd kept it simple. He also mentioned that Gojo's death and Kenjaku's exit were planned from the very beginning.